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SOX10 Antibody (Center)

APR11289G Product Datasheet Protocol

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APR11289G		100 μl / $495

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Leading Biology Inc.

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SOX10 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR11289G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SOX10 antibody (Center).
Molecular Weight	49911 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus.
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	231-259 aa
Target	This SOX10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-259 amino acids from the Central region of human SOX10.
Isotype	Rabbit Ig
GeneID	6663
UniProt ID	P56693
Function	Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia (By similarity).
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	IHC-P~~1:10~50 WB~~1:1000
Synonyms	Transcription factor SOX-10, SOX10
Images	APR11289G staining SOX10 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. APR11289G staining SOX10 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-SOX10 Antibody (Center) at 1:2000 dilution Lane 1: A375 whole cell lysates Lane 2: C6 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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