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SLC10A1 Antibody (C-term)

APR09991G Product Datasheet Protocol

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APR09991G		100 μl / $495

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Leading Biology Inc.

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SLC10A1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR09991G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SLC10A1 antibody (C-term).
Molecular Weight	38119 Da
Cellular Localization	Antigen Cellular Localization: Membrane; Multi-pass membrane protein.
Host	Rabbit
Species Reactivity	Human
Immunogen	316-343 aa
Target	This SLC10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-343 amino acids from the C-terminal region of human SLC10A1.
Isotype	Rabbit Ig
Symbol	NTCP
GeneID	6554
UniProt ID	Q14973
Function	The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
Summary	Sodium/bile acid cotransporters are integral membraneglycoproteins that participate in the enterohepatic circulation ofbile acids. Two homologous transporters are involved in thereabsorption of bile acids, one absorbing from the intestinallumen, the bile duct, and the kidney with an apical localization(SLC10A2; MIM 601295), and the other being found in the basolateralmembranes of hepatocytes (SLC10A1).
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, E
Dilution	FC~~1:25 WB~~1:2000
Synonyms	Sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1, SLC10A1, NTCP
Images	Overlay histogram showing K562 cells stained with APR09991G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR09991G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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