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ENOB Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR07706G 100 μl / $495

ENOB Antibody (Center)

Brand

Leading Biology

Catalog Number

APR07706G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ENOB antibody (Center).

Molecular Weight

46987 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity)

Host

Rabbit

Species Reactivity

Human

Immunogen

237-264 aa

Target

This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Appears to have a function in striated muscle development and regeneration.

Summary

ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, MSE, Skeletal muscle enolase, ENO3

Images

ENOB Antibody (Center) (Cat. APR07706G) western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).This demonstrates the ENOB antibody detected the ENOB protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ENOB Antibody (Center) (Cat. APR07706G) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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