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ENOA Antibody (N-term)

APR07705G Product Datasheet Protocol

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APR07705G		100 μl / $495

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ENOA Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR07705G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ENOA antibody (N-term).
Molecular Weight	47169 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	33-60 aa
Target	This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human ENOA.
Isotype	Rabbit Ig
Symbol	ENO1L1, MBPB1, MPB1
GeneID	2023
UniProt ID	P06733
Function	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Summary	ENO1 is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, IF, E
Dilution	WB~~1:1000 FC~~1:10~50 IF~~1:10~50
Synonyms	Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
Images	ENOA Antibody (N-term) (Cat. APR07705G) western blot analysis in U251,mouse C2C12,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow). ENOA Antibody (N-term) (Cat. APR07705G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term)( APR07705G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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