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Mouse Srms Antibody (Center)

APR17448G Product Datasheet Protocol

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APR17448G		100 μl / $495

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Leading Biology Inc.

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Mouse Srms Antibody (Center)

Brand	Leading Biology	Catalog Number	APR17448G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Mouse Srms antibody (Center).
Molecular Weight	55758 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Note=Localizes to punctate cytoplasmic structures.
Host	Rabbit
Species Reactivity	Mouse
Target	This Mouse Srms antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 113-147 amino acids from the Central region of Mouse Srms.
Isotype	Rabbit Ig
Symbol	Srm
UniProt ID	Q62270
Function	Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. May be involved in proliferation or differentiation of keratinocytes in the skin.
Summary	Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. May be involved in proliferation or differentiation of keratinocytes in the skin.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	IF~~1:25 WB~~1:1000
Synonyms	Tyrosine-protein kinase Srms, PTK70, Srms, Srm
Images	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized mouse skin tissue labeling Pdx1 with APR17448G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasm staining on mouse skin tissue. The nuclear counter stain is DAPI (blue). Anti-Srms Antibody (C-term) at 1:2000 dilution + mouse liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of lysates from mouse liver, mouse spleen tissue lysate (from left to right), using Srms Antibody (Center)( APR17448G). APR17448G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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