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(Mouse) Scx Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR14740G 100 μl / $495

(Mouse) Scx Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR14740G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Scx antibody (N-term).

Molecular Weight

22239 Da

Cellular Localization

Antigen Cellular Localization: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}

Host

Rabbit

Species Reactivity

Mouse

Target

This mouse Scx antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 69-102 amino acids from the N-terminal region of mouse Scx.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

Summary

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

IHC-P~~1:25 WB~~1:1000

Synonyms

Basic helix-loop-helix transcription factor scleraxis, Scx

Images

APR14740G staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of lysate from mouse lung tissue lysate, using Scx Antibody (N-term)( APR14740G). APR14740G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Specification

Quantity

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