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IL18 Antibody (C-term)

APR07981G Product Datasheet Protocol

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APR07981G		100 μl / $495

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Leading Biology Inc.

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IL18 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR07981G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality IL18 antibody (C-term).
Molecular Weight	22326 Da
Cellular Localization	Antigen Cellular Localization: Secreted.
Host	Rabbit
Species Reactivity	Human
Target	This IL18 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 155-188 amino acids from the C-terminal region of human IL18.
Isotype	Rabbit Ig
Symbol	IGIF, IL1F4
GeneID	3606
UniProt ID	Q14116
Function	Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Summary	Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, IF, IHC-P, E
Dilution	IHC-P~~1:25 IF~~1:25 FC~~1:25 WB~~1:1000
Synonyms	Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, IL18, IGIF, IL1F4
Images	Immunohistochemical analysis of paraffin-embedded H. small intestine section using IL18 Antibody (C-term)( APR07981G). APR07981G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Fluorescent image of U251 cells stained with IL18 Antibody (C-term) ( APR07981G). APR07981G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Flow cytometric analysis of Hela cells using IL18 Antibody (C-term)(green, APR07981G) compared to an isotype control of rabbit IgG(blue). APR07981G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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