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RSBN1 Antibody (N-term)

AMM07670G Product Datasheet Protocol

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AMM07670G		100 μl / $495

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Leading Biology Inc.

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RSBN1 Antibody (N-term)

Brand	Leading Biology	Catalog Number	AMM07670G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RSBN1 antibody (N-term).
Molecular Weight	90072 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Human
Immunogen	51-78 aa
Target	This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
Isotype	Rabbit Ig
GeneID	54665
UniProt ID	Q5VWQ0
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, FC, E
Dilution	IHC-P~~1:10~50 WB~~1:1000 FC~~1:10~50
Synonyms	Round spermatid basic protein 1, RSBN1
Images	AMM07670G staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. AMM07670G staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: MCF-7 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 90 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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