Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > DHCR7 Antibody (C-term)

DHCR7 Antibody (C-term)

APR07556G Product Datasheet Protocol

Catalog #	Availability	Size / Price	Inquiry
APR07556G		100 μl / $495

Bulk Orders Inquiry

3 Images

Contact Information

Leading Biology Inc.

tel:1-661-524(LBI)-0262
Info@leadingbiology.com

Show All Distributors

DHCR7 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR07556G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DHCR7 antibody (C-term).
Molecular Weight	54489 Da
Cellular Localization	Antigen Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	437-463 aa
Target	This DHCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.
Isotype	Rabbit Ig
Symbol	D7SR
GeneID	1717
UniProt ID	Q9UBM7
Function	Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
Summary	This gene encodes an enzyme that removes the C(7-8) doublebond in the B ring of sterols and catalyzes the conversion of7-dehydrocholesterol to cholesterol. This gene is ubiquitouslyexpressed and its transmembrane protein localizes to theendoplasmic reticulum membrane and nuclear outer membrane.Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); asyndrome that is metabolically characterized by reduced serumcholesterol levels and elevated serum 7-dehydrocholesterol levelsand phenotypically characterized by mental retardation, facialdysmorphism, syndactyly of second and third toes, andholoprosencephaly in severe cases to minimal physical abnormalitiesand near-normal intelligence in mild cases. Alternative splicingresults in multiple transcript variants that encode the sameprotein.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, IHC-P, E
Dilution	IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100
Synonyms	7-dehydrocholesterol reductase, 7-DHC reductase, Putative sterol reductase SR-2, Sterol Delta(7)-reductase, DHCR7, D7SR
Images	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP11452B at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Li-7 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human testis lysate Lane 5: mouse testis lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST. DHCR7 Antibody (C-term) (Cat. APR07556G) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the DHCR7 antibody detected the DHCR7 protein (arrow).
Specification	Product Datasheet Protocol
Quantity

Add to cart Inquiry

Previous Product: ALDH6A1 Polyclonal Antibody Next Product: p53 Antibody (S15)

Best Sellers

Related Products

Select	Brand	Catalog No.	Product Name	Pack Size	Type	Field of Research	Specification	Quantity	Price(USD)
1	Leading Biology	APR03440G	ITGA11 Antibody (N-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
2	Leading Biology	APR04537G	CMIP Antibody (C-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
3	Leading Biology	APR12422G	Human H4 Histamine Receptor (extracellular) Antibody	50 μl	Polyclonal Antibodies				$695.00	Add Ask
4	Leading Biology	APR03844G	UBE2W Antibody (C-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
5	Leading Biology	APR04349G	HECTD2 Antibody (N-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
6	Leading Biology	APR03502G	IGHG1 Antibody (Center)	100 μl	Polyclonal Antibodies				$495.00	Add Ask