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HSD17B3 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR07808G 100 μl / $495

HSD17B3 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR07808G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HSD17B3 antibody (Center).

Molecular Weight

34516 Da

Host

Rabbit

Species Reactivity

Human

Immunogen

89-118 aa

Target

This HSD17B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the Central region of human HSD17B3.

Isotype

Rabbit Ig

Symbol

EDH17B3

GeneID

UniProt ID

Function

Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.

Summary

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50

Synonyms

Testosterone 17-beta-dehydrogenase 3, 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, Testicular 17-beta-hydroxysteroid dehydrogenase, HSD17B3, EDH17B3

Images

All lanes : Anti-HSD17B3 Antibody (Center) at 1:1000 dilution Lane 1: human testis lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human testis tissue reacted with HSD17B3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

HSD17B3 Antibody (Center) (Cat. APR07808G) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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