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GBE1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
AMM04720G 100 μl / $495

GBE1 Antibody (Center)

Brand

Leading Biology

Catalog Number

AMM04720G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GBE1 antibody (Center).

Molecular Weight

80474 Da

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

527-556 aa

Target

This GBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-556 amino acids from the Central region of human GBE1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells.

Summary

GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

WB~~1:1000 IF~~1:10~50

Synonyms

4-alpha-glucan-branching enzyme, Brancher enzyme, Glycogen-branching enzyme, GBE1

Images

Western blot analysis of GBE1 Antibody (Center) (Cat. AMM04720G) in HL-60 cell line lysates (35ug/lane). GBE1 (arrow) was detected using the purified Pab;

Western blot analysis of GBE1 Antibody (Center) (Cat. AMM04720G) in mouse liver tissue lysates (35ug/lane).GBE1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of GBE1 Antibody (Center)( AMM04720G) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Specification

Quantity

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