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Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term)

AMM04615G Product Datasheet Protocol

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AMM04615G		100 μl / $495

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Leading Biology Inc.

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Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM04615G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Fructose 6 Phosphate Kinase (PFKM) antibody (C-term).
Molecular Weight	85183 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_03184}.
Host	Rabbit
Species Reactivity	Human
Immunogen	746-776 aa
Target	This Fructose 6 Phosphate Kinase (PFKM) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 746-776 amino acids from the C-terminal region of human Fructose 6 Phosphate Kinase (PFKM).
Isotype	Rabbit Ig
Symbol	PFKX
GeneID	5213
UniProt ID	P08237
Function	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Summary	Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 IF~~1:10~50
Synonyms	ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255\|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255\|HAMAP-Rule:MF_03184}, PFK-M, 27111 {ECO:0000255\|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohexokinase {ECO:0000255\|HAMAP-Rule:MF_03184}, PFKM, PFKX
Images	Western blot analysis of PFKM polyclonal antibody (Cat. AMM04615G) in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term)(Cat. AMM04615G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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