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Fructose 6 Phosphate Kinase Antibody (N-term)

AMM04616G Product Datasheet Protocol

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AMM04616G		100 μl / $495

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Leading Biology Inc.

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Fructose 6 Phosphate Kinase Antibody (N-term)

Brand	Leading Biology	Catalog Number	AMM04616G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Fructose 6 Phosphate Kinase antibody (N-term).
Molecular Weight	85183 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_03184}.
Host	Rabbit
Species Reactivity	Human
Immunogen	122-151 aa
Target	This Fructose 6 Phosphate Kinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the N-terminal region of human Fructose 6 Phosphate Kinase.
Isotype	Rabbit Ig
Symbol	PFKX
GeneID	5213
UniProt ID	P08237
Function	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Summary	Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:50~100
Synonyms	ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255\|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255\|HAMAP-Rule:MF_03184}, PFK-M, 27111 {ECO:0000255\|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohexokinase {ECO:0000255\|HAMAP-Rule:MF_03184}, PFKM, PFKX
Images	Western blot analysis of lysate from human skeletal muscle tissue lysate, using PFKM Antibody （S137）(Cat. AMM04616G). AMM04616G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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