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PCK1 Antibody (C-term)

APR07004G Product Datasheet Protocol

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APR07004G		100 μl / $495

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Leading Biology Inc.

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PCK1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR07004G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PCK1 antibody (C-term).
Molecular Weight	69195 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	592-622 aa
Target	This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.
Isotype	Rabbit Ig
Symbol	PEPCK1
GeneID	5105
UniProt ID	P35558
Function	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Summary	This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100
Synonyms	Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1
Images	Western blot analysis of PCK1 Antibody (C-term) (Cat. APR07004G) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab. PCK1 Antibody (C-term) (Cat. APR07004G) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PCK1 antibody detected the PCK1 protein (arrow). Confocal immunofluorescent analysis of PCK1 Antibody (C-term)( APR07004G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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