> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > PCK1 Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR08951G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PCK1 antibody (N-term).
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Molecular Weight |
69195 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm.
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
40-70 aa
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Target |
This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the N-terminal region of human PCK1.
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Isotype |
Rabbit Ig
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Symbol |
PEPCK1
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GeneID |
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UniProt ID |
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Function |
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
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Summary |
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
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Synonyms |
Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1
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Images |
The anti-PCK1 Pab (Cat. APR08951G) is used in Western blot to detect PCK1 in mouse liver tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PCK1 Antibody (N-term) (Cat. APR08951G) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCK1 antibody detected the PCK1 protein (arrow). |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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