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EHD3 Antibody (N-term)

AMM07022G Product Datasheet Protocol

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AMM07022G		100 μl / $495

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Leading Biology Inc.

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EHD3 Antibody (N-term)

Brand	Leading Biology	Catalog Number	AMM07022G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EHD3 antibody (N-term).
Molecular Weight	60887 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein Recycling endosome membrane; Peripheral membrane protein Cytoplasm
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	22-48 aa
Target	This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human EHD3.
Isotype	Rabbit Ig
Symbol	EHD2, PAST3
GeneID	30845
UniProt ID	Q9NZN3
Function	Plays a role in endocytic transport.
Summary	EHD3 plays a role in endocytic transport.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
Synonyms	EH domain-containing protein 3, PAST homolog 3, EHD3, EHD2, PAST3
Images	Anti-EHD3 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of EHD3 Antibody (N-term) (Cat. AMM07022G) in mouse lung tissue lysates (35ug/lane).EHD3 (arrow) was detected using the purified Pab. EHD3 Antibody (N-term) (Cat. AMM07022G) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EHD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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