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EN2 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR11821G 100 μl / $495

EN2 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR11821G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EN2 antibody (C-term).

Molecular Weight

34211 Da

Cellular Localization

Antigen Cellular Localization: Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

243-271 aa

Target

This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.

Isotype

Rabbit Ig

GeneID

UniProt ID

Summary

EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2

Images

Western blot analysis of EN2 Antibody (C-term) (Cat. APR11821G) in mouse cerebellum, brain tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with EN2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EN2 Antibody (C-term) (Cat. APR11821G) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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