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EN2 Antibody (C-term)

APR11822G Product Datasheet Protocol

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APR11822G		100 μl / $495

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Leading Biology Inc.

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EN2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR11822G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EN2 antibody (C-term).
Molecular Weight	34211 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	243-271 aa
Target	This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
Isotype	Rabbit Ig
GeneID	2020
UniProt ID	P19622
Summary	EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	WB~~1:1000 IF~~1:10~50
Synonyms	Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2
Images	Anti-EN2 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-EN2Antibody(C-term)at 1:1000 dilution + Neuro-2a whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent confocal image of Hela cell stained with EN2 Antibody (C-term)( APR11822G). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
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