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AQP1 Antibody

Catalog # Availability Size / Price Inquiry
APG01959G 100 μl / $495

AQP1 Antibody

Brand

Leading Biology

Catalog Number

APG01959G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AQP1 antibody.

Molecular Weight

28; [uniprot]28,20,23,17; KDa

Cellular Localization

Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Dog, Sheep

Immunogen

181-269/269 aa

Symbol

CHIP28

GeneID

UniProt ID

Function

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Summary

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Form

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB

Dilution

WB~~1:100~1:500 IHC-P~~1:100~1:500

Synonyms

Aquaporin-1, AQP-1, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, AQP1, CHIP28

Images

Lane 1: mouse heart lysates Lane 2: mouse lung lysates probed with Anti AQP1/CHIP Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 in 4?C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37?C. Predicted band 28kD. Observed band size: 28kD.

Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (APG01959G) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-AF488) at [1:500] for 90 minutes and DAPI staining of the nuclei.

Specification

Quantity

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