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USO1 Antibody (C-term)

APR14417G Product Datasheet Protocol

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APR14417G		100 μl / $495

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Leading Biology Inc.

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USO1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR14417G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality USO1 antibody (C-term).
Molecular Weight	107895 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes
Host	Rabbit
Species Reactivity	Human
Immunogen	932-960 aa
Target	This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1.
Isotype	Rabbit Ig
Symbol	VDP
GeneID	8615
UniProt ID	O60763
Function	General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity).
Summary	The protein encoded by this gene is a peripheral membraneprotein which recycles between the cytosol and the Golgi apparatusduring interphase. It is regulated by phosphorylation:dephosphorylated protein associates with the Golgi membrane anddissociates from the membrane upon phosphorylation. Ras-associatedprotein 1 recruits this protein to coat protein complex II (COPII)vesicles during budding from the endoplasmic reticulum, where itinteracts with a set of COPII vesicle-associated SNAREs to form acis-SNARE complex that promotes targeting to the Golgi apparatus.Transport from the ER to the cis/medial Golgi compartments requiresthe action of this gene product, GM130 and giantin in a sequentialmanner.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Synonyms	General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP
Images	USO1 Antibody (C-term) (Cat. APR14417G) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the USO1 antibody detected the USO1 protein (arrow). USO1 Antibody (C-term) (APR14417G)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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