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GSTT1 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
AMM05271G 100 μl / $495

GSTT1 Antibody (N-term)

Brand

Leading Biology

Catalog Number

AMM05271G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GSTT1 antibody (N-term).

Molecular Weight

27335 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

7-34 aa

Target

This GSTT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human GSTT1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

Summary

Glutathione S-transferase (GST) theta 1 (GSTT1) is amember of a superfamily of proteins that catalyze the conjugationof reduced glutathione to a variety of electrophilic andhydrophobic compounds. Human GSTs can be divided into five mainclasses: alpha, mu, pi, theta, and zeta. The theta class includesGSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequenceidentity and both of them were claimed to have an important role inhuman carcinogenesis. The GSTT1 gene is located approximately 50kbaway from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similarstructure, being composed of five exons with identical exon/intronboundaries.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, E

Dilution

WB~~1:1000 FC~~1:10~50

Synonyms

Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1, GSTT1

Images

Anti-GSTT1 Antibody (N-term)at 1:2000 dilution + human lung lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GSTT1 (arrow) using rabbit polyclonal GSTT1 Antibody (N-term) (Cat. AMM05271G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTT1 gene.

GSTT1 Antibody (N-term) (Cat. AMM05271G) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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