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RASGRP2 Antibody (N-term)

AMM07541G Product Datasheet Protocol

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AMM07541G		100 μl / $495

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Leading Biology Inc.

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RASGRP2 Antibody (N-term)

Brand	Leading Biology	Catalog Number	AMM07541G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RASGRP2 antibody (N-term).
Molecular Weight	69248 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity)
Host	Rabbit
Species Reactivity	Human
Immunogen	7-34 aa
Target	This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2.
Isotype	Rabbit Ig
Symbol	CDC25L, MCG7
GeneID	10235
UniProt ID	Q7LDG7
Function	Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
Summary	The protein encoded by this gene is a brain-enrichednucleotide exchanged factor that contains an N-terminal GEF domain,2 tandem repeats of EF-hand calcium-binding motifs, and aC-terminal diacylglycerol/phorbol ester-binding domain. Thisprotein can activate small GTPases, including RAS and RAP1/RAS3.The nucleotide exchange activity of this protein can be stimulatedby calcium and diacylglycerol. Three alternatively splicedtranscript variants encoding the same protein have been found forthis gene.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
Synonyms	RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, Cdc25-like protein, hCDC25L, F25B33 kinase-like protein, RASGRP2, CDC25L, MCG7
Images	Anti-RASGRP2 Antibody (N-term) at 1:1000 dilution + human spleen lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST. RASGRP2 Antibody (N-term) (Cat. AMM07541G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow). RASGRP2 Antibody (N-term) (Cat. AMM07541G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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