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PCYT1A Antibody (N-term)

APR12776G Product Datasheet Protocol

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APR12776G		100 μl / $495

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Leading Biology Inc.

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PCYT1A Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR12776G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PCYT1A antibody (N-term).
Molecular Weight	41731 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Note=It can interconvert between an inactive cytosolic form and an active membrane-bound form.
Host	Rabbit
Species Reactivity	Human
Immunogen	27-54 aa
Target	This PCYT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human PCYT1A.
Isotype	Rabbit Ig
Symbol	CTPCT, PCYT1
GeneID	5130
UniProt ID	P49585
Function	Controls phosphatidylcholine synthesis.
Summary	PCYT1A controls phosphatidylcholine synthesis.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
Synonyms	Choline-phosphate cytidylyltransferase A, CCT-alpha, CTP:phosphocholine cytidylyltransferase A, CCT A, CT A, Phosphorylcholine transferase A, PCYT1A, CTPCT, PCYT1
Images	All lanes : Anti-PCYT1A Antibody (N-term) at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST. PCYT1A Antibody (N-term) (Cat. APR12776G)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCYT1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Confocal immunofluorescent analysis of PCYT1A Antibody (N-term)( APR12776G) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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