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Calmodulin Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR10831G 100 μl / $545

Calmodulin Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR10831G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Calmodulin antibody (C-term).

Molecular Weight

16838 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules

Host

Rabbit

Species Reactivity

Human

Immunogen

117-149 aa

Target

This Calmodulin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-149 amino acids from the C-terminal region of human Calmodulin.

Isotype

Rabbit Ig

Symbol

CALM, CAM, CAM1

GeneID

UniProt ID

Function

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

Summary

Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Synonyms

Calmodulin, CaM, CALM1, CALM, CAM, CAM1

Images

Western blot analysis of anti-Calmodulin Antibody (C-term)(Cat. APR10831G) in Hela cell line lysates (35ug/lane). Calmodulin(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with Calmodulin antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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