We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Sphingosine 1-Phosphate Receptor 3 (extracellular) Antibody.

42270 Da

Rabbit

Human, Mouse, Rat

Peptide (C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse Sphingosine 1-phosphate receptor 3 (Accession Q9Z0U9). Extracellular, N-terminus.

Sphingosine 1-phosphate (S1P) is an active byproduct of sphingomyelin metabolism. All cells can synthesize this biomolecule but the majority of its synthesis comes from erythrocytes and endothelial cells. In cases of inflammation, mast cells and platelets are the main source of sphingosine 1-phosphate1. S1P cellular functions could be intracellular, where it is synthesized. In addition, upon secretion, it circulates in the blood via its binding to high-density lipoproteins and albumin. When S1P reaches its target cell it could activate 5 different high affinity receptors belonging to the G-protein coupled receptor superfamily: Sphingosine 1-phosphate receptors, termed S1PR1-5 1.Stimulation of S1PR receptors triggers a cascade of signaling events depending on the receptor and on the G-protein it couples. S1PR1 couples to Gi2. S1PR2 can couple to Gs, Gq or G12/13 and S1PR3-5 can couple to Gi or G12/133-4. The pathways activated vary from Ca2+ mobilization, activation or inhibition of adenylate cyclase phospholipase C activation and more. Through the different signaling pathways these receptors activate, S1P1R receptors are implicated in adherens junction assembly, cytoskeletal changes, cell migration, proliferation and apoptosis5. S1PR3 is specifically detected in the brain, heart, spleen, liver, lung, thymus, kidney, testis and skeletal muscle3. S1PR1 and S1PR2 are generally expressed in the CNS, cardiovascular and immune systems1. S1PR4 is specifically expressed in the lymphoid tissue and S1PR5 in natural killer cells and olygodendrocytes1.Malfunction of S1PR receptor signaling is reported in various disorders, for example multiple sclerosis and may be targets for the development of therapeutic drugs1.Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse sphingosine 1-phosphate receptor 3. Anti-Sphingosine 1-Phosphate Receptor 3 (extracellular) antibody (#AG1013) can be used in western blot analysis and indirect flow cytometry applications. It has been designed to recognize S1PR3 from human, rat and mouse samples.

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

FC, WB

FC~~1:40 WB~~1:200-1:2000

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