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HSP90B Antibody (Ab-254)

Catalog # Availability Size / Price Inquiry
APR05371G 100 μl / $495

HSP90B Antibody (Ab-254)

Brand

Leading Biology

Catalog Number

APR05371G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HSP90B Antibody (Ab-254) .

Molecular Weight

84 KDa

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

243-272 aa

Symbol

HSP90B, HSPC2, HSPCB

GeneID

UniProt ID

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Summary

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, IHC

Dilution

WB~~ 1:1000 IF~~1:100 IHC~~1:50-1:100

Images

Western blot analysis of lysates from Hela,A431 cell line (from left to right),using HSP90B Antibody (Ab-254) (B0013). B0013 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Western blot analysis of extracts from CoLo cells (Lane 1), HepG2 cells (Lane 2) and 293 cells (Lane 3), using HSP90B (Ab-254) Antibody. The lane on the left is treated with synthesized peptide.

Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (Ab-254) antibody .

Specification

Quantity

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