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Vimentin Antibody (S82)

APR05062G Product Datasheet Protocol

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APR05062G		100 μl / $495

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Leading Biology Inc.

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Vimentin Antibody (S82)

Brand	Leading Biology	Catalog Number	APR05062G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Vimentin Antibody (S82).
Molecular Weight	53652 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	63-90 aa
Target	This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.
Isotype	Rabbit Ig
GeneID	7431
UniProt ID	P08670
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Summary	Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, IHC-P, E
Dilution	IF~~1:200 WB~~1:1000 IHC-P~~1:10~50
Images	Fluorescent confocal image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APR05062G Vimentin (S82) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025). Western blot analysis of Vimentin-S82 (Cat. APR05062G) in Hela cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab. Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) (Cat.APR05062G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
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