We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GABA(A) alpha4 (extracellular) antibody.

60951 Da

Rabbit

Human, Mouse, Rat

Peptide NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of ??rat ??GABA(A) ?±4 with replacement of C44 with serine (Accession ??P28471 ). Extracellular, N-terminus.

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) α4 subunit. Anti-GABA(A) α4 Receptor (extracellular) antibody (#AG1272) can be used in western blot and immunohistochemistry applications. It was designed to recognize GABA(A) α4 from human, rat and mouse samples.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB

WB~~1:200-1:2000

Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4, Gabra4

Product Datasheet Protocol