We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Human CysLTR2 (extracellular) antibody.

39635 Da

Rabbit

Human

Peptide (C)HLTTWKVGL(S)KDRLH, corresponding to amino acid residues 270-284 of human CysLTR2 (Accession Q9NS75). 3rd extracellular loop.Not recommended for use with rat and mouse samples.

LTC4, LTD4, and LTE4are cysteinyl leukotrienes (cys-LTs) peptide conjugated lipids derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1and cysLT2respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o)and insensitive (Gq, which initiates Ca2+influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4and binds equally to LTC4and LTE4whereas CysLTR2 has greater and equal affinity towards LTC4and LTD4over LTE45.Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14.Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6.Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of humanCysLTR2.Anti-CysLTR2 (extracellular)antibody (#AG1221) can be used for western blot analysisand indirect flow cytometry applications.It has been designed to recognize CysLTR2 from human samples only.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB

WB~~1:200-1:2000 FC~~1:50

Cysteinyl leukotriene receptor 2, CysLTR2, G-protein coupled receptor GPCR21, hGPCR21, G-protein coupled receptor HG57, HPN321, CYSLTR2, CYSLT2, CYSLT2R

Product Datasheet Protocol