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PDIA3 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
AMM07073G 100 μl / $495

PDIA3 Antibody (C-term)

Brand

Leading Biology

Catalog Number

AMM07073G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PDIA3 antibody (C-term).

Molecular Weight

H=57;M=57;R=57 KDa

Cellular Localization

Antigen Cellular Localization: Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

446-475 aa

Target

This PDIA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-475 amino acids from the C-terminal region of human PDIA3.

Symbol

ERP57, ERP60, GRP58

GeneID

UniProt ID

Summary

PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Form

Liquid. In PBS containing 0.02% sodium azide.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

FC, IHC-P, WB

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3, ERP57, ERP60, GRP58

Images

All lanes : Anti-PDIA3 Antibody (C-term) at 1:1000 dilution Lane 1: A2058 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: 293T/17 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PDIA3 Antibody (C-term) (Cat. AMM07073G) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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