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LDHA Antibody (C-term)

APR08176G Product Datasheet Protocol

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APR08176G		100 μl / $495

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Leading Biology Inc.

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LDHA Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR08176G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality LDHA antibody (C-term).
Molecular Weight	H=40,30,27,37;M=36;R=36 KDa
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	204-232 aa
Target	This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA.
GeneID	3939
UniProt ID	P00338
Summary	The protein encoded by this gene catalyzes the conversionof L-lactate and NAD to pyruvate and NADH in the final step ofanaerobic glycolysis. The protein is found predominantly in muscletissue and belongs to the lactate dehydrogenase family. Mutationsin this gene have been linked to exertional myoglobinuria. Multipletranscript variants encoding different isoforms have been found forthis gene. The human genome contains several non-transcribedpseudogenes of this gene.
Form	Liquid. In PBS containing 0.02% sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, IHC-P
Dilution	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
Synonyms	L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
Images	All lanes : Anti-LDHA Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: human placenta lysate Lane 4: PC-3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST. Confocal immunofluorescent analysis of LDHA Antibody (C-term)( APR08176G) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). LDHA Antibody (C-term) (Cat. APR08176G)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LDHA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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