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Mouse Klf4 Antibody (Center)

APR17828G Product Datasheet Protocol

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APR17828G		100 μl / $495

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Leading Biology Inc.

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Mouse Klf4 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR17828G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Mouse Klf4 antibody (Center).
Molecular Weight	M=52 KDa
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Mouse
Immunogen	321-354 aa
Target	This Mouse Klf4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 321-354 amino acids from the Central region of mouse Klf4.
Symbol	Ezf, Gklf, Zie
GeneID	16600
UniProt ID	Q60793
Function	Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).
Summary	Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC
Dilution	FC~~1:25 WB~~1:1000
Synonyms	Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, Klf4, Ezf, Gklf, Zie
Images	Overlay histogram showing NIH/3T3 cells stained with APR17828G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR17828G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-Klf4 Antibody (Center) at 1:1000 dilution Lane 1: mouse heart lysates Lane 2: mouse small intestine lysates Lane 3: mouse testis lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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