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DMRTA2 Antibody (C-term)

APC00106G Product Datasheet Protocol

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APC00106G		100 μl / $495

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Leading Biology Inc.

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DMRTA2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APC00106G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DMRTA2 antibody (C-term).
Molecular Weight	H=53;M=53;Z=47 KDa
Cellular Localization	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00070}
Host	Rabbit
Species Reactivity	Human, Mouse, Zebrafish
Immunogen	487-515 aa
Target	This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Symbol	DMRT5
GeneID	63950
UniProt ID	Q96SC8
Function	May be involved in sexual development.
Summary	DMRTA2 may be involved in sexual development.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF
Dilution	WB~~1:1000 IF~~1:10~50
Synonyms	DMRTA2; DMRT5; Doublesex- and mab-3-related transcription factor A2; Doublesex- and mab-3-related transcription factor 5
Images	Western blot analysis of lysates from K562 cell line,zebra fish brain,mouse brain,mouse testis tissue lysate,PC-3 cell line (from left to right), using DMRTA2 Antibody (C-term)(Cat. APC00106G). APC00106G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane. Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)( APC00106G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
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