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TYSY Antibody(C-term)

APR10627G Product Datasheet Protocol

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APR10627G		100 μl / $495

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Leading Biology Inc.

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TYSY Antibody(C-term)

Brand	Leading Biology	Catalog Number	APR10627G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TYSY antibody(C-term).
Molecular Weight	35716 Da
Cellular Localization	Antigen Cellular Localization: Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane
Host	Rabbit
Species Reactivity	Human
Immunogen	265-294 aa
Target	This TYSY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human TYSY.
Clone	RB20041
Isotype	Rabbit Ig
Symbol	TS
GeneID	7298
UniProt ID	P04818
Function	Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Summary	Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, FC, E
Dilution	WB~~1:2000 IHC-P~~1:500 IF~~1:50 FC~~1:50
Synonyms	Thymidylate synthase, TS, TSase, TYMS, TS
Images	TYSY Antibody (C-term) (Cat. APR10627G) western blot analysis in Hela,Jurkat and HL-60 cell line lysates (35ug/lane).This demonstrates the TYSY antibody detected the TYSY protein (arrow). TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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