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CDH20 Antibody (N-term)

APR15398G Product Datasheet Protocol

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APR15398G		100 μl / $495

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Leading Biology Inc.

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CDH20 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR15398G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CDH20 antibody (N-term).
Molecular Weight	88993 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Single-pass type I membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	111-140 aa
Target	This CDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human CDH20.
Isotype	Rabbit Ig
Symbol	CDH7L3
GeneID	28316
UniProt ID	Q9HBT6
Function	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Summary	This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Synonyms	Cadherin-20, CDH20, CDH7L3
Images	Western blot analysis of lysates from human brain and mouse brain tissue lysate(from left to right), using CDH20 Antibody (N-term)( APR15398G). APR15398G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of CDH20 Antibody (N-term) (Cat. APR15398G) in HepG2 cell line lysates (35ug/lane). CDH20 (arrow) was detected using the purified Pab. CDH20 Antibody (N-term) (Cat. APR15398G) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDH20 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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