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ADH7 Antibody (C-Term)

APR14853G Product Datasheet Protocol

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APR14853G		100 μl / $495

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ADH7 Antibody (C-Term)

Brand	Leading Biology	Catalog Number	APR14853G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ADH7 antibody (C-Term).
Molecular Weight	41481 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human
Immunogen	318-346 aa
Target	This ADH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the C-terminal region of human ADH7.
Isotype	Rabbit Ig
GeneID	131
UniProt ID	P40394
Function	Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism.
Summary	This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, IF, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50
Synonyms	Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Retinol dehydrogenase, ADH7
Images	Western blot analysis of lysates from HepG2, SW480 cell line (from left to right), using ADH7 Antibody (C-Term)( APR14853G). APR14853G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. ADH7 Antibody (C-Term) (Cat. APR14853G) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH7 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated. ADH7 Antibody (C-Term) (Cat. APR14853G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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