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GSTP1 Antibody (Center)

AMM05267G Product Datasheet Protocol

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AMM05267G		100 μl / $495

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GSTP1 Antibody (Center)

Brand	Leading Biology	Catalog Number	AMM05267G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GSTP1 antibody (Center).
Molecular Weight	23356 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Mitochondrion. Nucleus. Note=The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	97-126 aa
Target	This GSTP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-126 amino acids from the Central region of human GSTP1.
Isotype	Rabbit Ig
Symbol	FAEES3, GST3
GeneID	2950
UniProt ID	P09211
Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Summary	Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, FC, E
Dilution	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100 FC~~1:10~50
Synonyms	Glutathione S-transferase P, GST class-pi, GSTP1-1, GSTP1, FAEES3, GST3
Images	GSTP1 Antibody (Center) (Cat. AMM05267G) western blot analysis in K562,PC-3,Y79 cell line and mouse lung tissue lysates (35ug/lane).This demonstrates the GSTP1 antibody detected the GSTP1 protein (arrow). Fluorescent confocal image of A549 cell stained with GSTP1 Antibody (Center)( AMM05267G).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GSTP1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). GSTP1 immunoreactivity is localized to Cytoplasm and Mitochondria significantly. Fluorescent confocal image of A549 cell stained with GSTP1 Antibody (Center)( AMM05267G).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GSTP1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). GSTP1 immunoreactivity is localized to Cytoplasm significantly.
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