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OAT Antibody (N-term)

APR08880G Product Datasheet Protocol

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APR08880G		100 μl / $495

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Leading Biology Inc.

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OAT Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR08880G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality OAT antibody (N-term).
Molecular Weight	48535 Da
Cellular Localization	Antigen Cellular Localization: Mitochondrion matrix
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	27-55 aa
Target	This OAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human OAT.
Isotype	Rabbit Ig
GeneID	4942
UniProt ID	P04181
Summary	OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, IF, E
Dilution	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50 FC~~1:10~50
Synonyms	Ornithine aminotransferase, mitochondrial, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, Ornithine aminotransferase, hepatic form, Ornithine aminotransferase, renal form, OAT
Images	All lanes : Anti-OAT Antibody (N-term) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST. OAT Antibody (N-term) (Cat. APR08880G) western blot analysis in 293,293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane).This demonstrates the OAT antibody detected the OAT protein (arrow). Fluorescent image of A549 cell stained with OAT Antibody (N-term)( APR08880G/SA100310AG).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with OAT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).OAT immunoreactivity is localized to Mitochondrion significantly.
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