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MSRA Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR08540G 100 μl / $495

MSRA Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR08540G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MSRA antibody (N-term).

Molecular Weight

26132 Da

Cellular Localization

Antigen Cellular Localization: Isoform 1: Mitochondrion. Isoform 3: Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

34-63 aa

Target

This MSRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human MSRA.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.

Summary

MSRA is ubiquitous and highly conserved. This protein carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The protein's proposed function is the repair of oxidative damage to proteins to restore biological activity.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, IF, E

Dilution

WB~~1:1000 IF~~1:100 IHC-P~~1:10~50 FC~~1:10~50

Synonyms

Mitochondrial peptide methionine sulfoxide reductase, Peptide-methionine (S)-S-oxide reductase, Peptide Met(O) reductase, Protein-methionine-S-oxide reductase, PMSR, MSRA

Images

Anti-MSRA Antibody (N-term) at 1:1000 dilution + human kidney lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of A549 cells, using MSRA Antibody (N-term) (Cat. APR08540G). APR08540G was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor? 554 (red) conjugated Phalloidin (red).

Western blot analysis of MSRA antibody (N-term) (Cat. APR08540G) in mouse kidney tissue lysates (35ug/lane). MSRA (arrow) was detected using the purified Pab.

Specification

Quantity

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