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PGK1 Antibody (Center S320)

Catalog # Availability Size / Price Inquiry
APR07144G 100 μl / $495

PGK1 Antibody (Center S320)

Brand

Leading Biology

Catalog Number

APR07144G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PGK1 antibody (Center S320).

Molecular Weight

44615 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

305-334 aa

Target

This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human PGK1.

Isotype

Rabbit Ig

Symbol

PGKA

GeneID

UniProt ID

Function

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).

Summary

Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Synonyms

Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA

Images

Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) ( APR07144G). APR07144G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 Antibody (Center S320)(Cat. APR07144G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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