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MOB4A Antibody (C-term)

APR14293G Product Datasheet Protocol

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APR14293G		100 μl / $495

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Leading Biology Inc.

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MOB4A Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR14293G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MOB4A antibody (C-term).
Molecular Weight	25091 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus
Host	Rabbit
Species Reactivity	Human
Immunogen	177-206 aa
Target	This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
Isotype	Rabbit Ig
Symbol	MOB4A, MOBKL1A
GeneID	92597
UniProt ID	Q7L9L4
Function	Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.
Summary	MOB4A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob4A regulates NDR1 and NDR2 kinase activity, widely expressed nuclear serine-threonine kinases that have been implicated in cell proliferation and/or tumor progression.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
Synonyms	MOB kinase activator 1B, Mob1 homolog 1A, Mob1A, Mob1B, Mps one binder kinase activator-like 1A, MOB1B, MOB4A, MOBKL1A
Images	Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term) ( APR14293G). APR14293G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. MOB4A Antibody (C-term) (Cat. APR14293G) western blot analysis in Daudi,K562 cell line lysates (35ug/lane).This demonstrates the MOB4A antibody detected the MOB4A protein (arrow). Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody (C-term)( APR14293G).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).MOB4A immunoreactivity is localized to Cytoplasm significantly.
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