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HSPD1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR16777G 100 μl / $495

HSPD1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR16777G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HSPD1 antibody (C-term).

Molecular Weight

61055 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion matrix.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

396-423 aa

Target

This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the C-terminal region of human HSPD1.

Isotype

Rabbit Ig

Symbol

HSP60

GeneID

UniProt ID

Function

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Summary

HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60

Images

HSPD1 Antibody (C-term) (Cat. APR16777G) western blot analysis in A431,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

HSPD1 Antibody (C-term) (Cat. APR16777G) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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