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HTR1E Antibody (C-Term)

AMM06012G Product Datasheet Protocol

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AMM06012G		100 μl / $495

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Leading Biology Inc.

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HTR1E Antibody (C-Term)

Brand	Leading Biology	Catalog Number	AMM06012G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HTR1E antibody (C-Term).
Molecular Weight	41682 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Multi- pass membrane protein
Host	Rabbit
Species Reactivity	Human
Target	This HTR1E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 223-258 amino acids from human HTR1E.
Isotype	Rabbit Ig
GeneID	3354
UniProt ID	P28566
Function	G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
Summary	G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, E
Dilution	IHC-P~~1:25 WB~~1:2000
Synonyms	5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E
Images	AMM06012G staining HTR1E in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-HTR1E Antibody (C-Term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: human brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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