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FEV Antibody (N-Term)

APR11904G Product Datasheet Protocol

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APR11904G		100 μl / $495

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Leading Biology Inc.

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FEV Antibody (N-Term)

Brand	Leading Biology	Catalog Number	APR11904G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality FEV antibody (N-Term).
Molecular Weight	25030 Da
Cellular Localization	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00237, ECO:0000269\|PubMed:12761502}
Host	Rabbit
Species Reactivity	Human
Target	This FEV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-49 amino acids from human FEV.
Isotype	Rabbit Ig
Symbol	PET1
GeneID	54738
UniProt ID	Q99581
Function	Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.
Summary	Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, E
Dilution	IHC-P~~1:25 WB~~1:2000
Synonyms	Protein FEV, Fifth Ewing variant protein, PC12 ETS domain-containing transcription factor 1, PC12 ETS factor 1, Pet-1, FEV, PET1
Images	APR11904G staining FEV in human small intestine sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-FEV Antibody (N-Term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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