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GNL3 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR17769G 100 μl / $495

GNL3 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR17769G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GNL3 antibody (N-term).

Molecular Weight

61993 Da

Cellular Localization

Antigen Cellular Localization: Nucleus. Nucleus, nucleolus. Note=Shuttles between the nucleus and nucleolus

Host

Rabbit

Species Reactivity

Human

Target

This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-124 amino acids from the N-terminal region of human GNL3.

Isotype

Rabbit Ig

Symbol

E2IG3, NS

GeneID

UniProt ID

Function

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

Summary

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

IF~~1:25 WB~~1:2000

Synonyms

Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS

Images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling GNL3 with APR17769G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing both nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor? 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).

All lanes : Anti-GNL3 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: NCCIT whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

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