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SNCA Antibody (C-term)

APR13431G Product Datasheet Protocol

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APR13431G		100 μl / $495

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Leading Biology Inc.

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SNCA Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR13431G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SNCA antibody (C-term).
Molecular Weight	14460 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Note=Membrane-bound in dopaminergic neurons
Host	Rabbit
Species Reactivity	Human, Mouse, Rat, Cynomolgus Monkey
Target	This SNCA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-125 amino acids from the C-terminal region of human SNCA.
Isotype	Rabbit Ig
Symbol	NACP, PARK1
GeneID	6622
UniProt ID	P37840
Function	May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Summary	May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, IHC-P, IHC, E
Dilution	IHC~~1:25 IHC-P~~1:25 FC~~1:25 WB~~1:1000
Synonyms	Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
Images	APR13431G staining SNCA in Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. APR13431G staining SNCA in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Flow cytometric analysis of HeLa cells using SNCA Antibody (C-term)(green, APR13431G) compared to an isotype control of rabbit IgG(blue). APR13431G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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