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STAT5A Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR10277G 100 μl / $495

STAT5A Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR10277G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality STAT5A antibody (C-term).

Molecular Weight

90647 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation

Host

Rabbit

Species Reactivity

Human

Target

This STAT5A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 730-765 amino acids from the C-terminal region of human STAT5A.

Isotype

Rabbit Ig

Symbol

STAT5

GeneID

UniProt ID

Function

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

Summary

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

IF~~1:25 WB~~1:1000

Synonyms

Signal transducer and activator of transcription 5A, STAT5A, STAT5

Images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling STAT5A with APR10277G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor? 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).

Western blot analysis of lysate from K562 cell line, using STAT5A Antibody (C-term)( APR10277G). APR10277G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Specification

Quantity

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