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PAFAH1B1 Antibody (N-term)

APR12705G Product Datasheet Protocol

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APR12705G		100 μl / $495

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Leading Biology Inc.

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PAFAH1B1 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR12705G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PAFAH1B1 antibody (N-term).
Molecular Weight	46638 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle {ECO:0000255\|HAMAP- Rule:MF_03141}. Nucleus membrane {ECO:0000255\|HAMAP- Rule:MF_03141}. Note=Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane.
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Target	This PAFAH1B1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 76-110 amino acids from the N-terminal region of human PAFAH1B1.
Isotype	Rabbit Ig
GeneID	5048
UniProt ID	P43034
Function	Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
Summary	Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	IHC-P~~1:25 WB~~1:1000
Synonyms	Platelet-activating factor acetylhydrolase IB subunit alpha {ECO:0000255\|HAMAP-Rule:MF_03141}, Lissencephaly-1 protein {ECO:0000255\|HAMAP-Rule:MF_03141}, LIS-1 {ECO:0000255\|HAMAP-Rule:MF_03141}, PAF acetylhydrolase 45 kDa subunit {ECO:0000255\|HAMAP-Rule:MF_03141}, PAF-AH 45 kDa subunit {ECO:0000255\|HAMAP-Rule:MF_03141}, PAF-AH alpha {ECO:0000255\|HAMAP-Rule:MF_03141}, PAFAH alpha {ECO:0000255\|HAMAP-Rule:MF_03141}, PAFAH1B1 {ECO:0000255\|HAMAP-Rule:MF_03141}
Images	Immunohistochemical analysis of paraffin-embedded H. brain section using PAFAH1B1 Antibody (N-term)( APR12705G). APR12705G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysates from HeLa, mouse C2C12, rat C6 cell line (from left to right), using PAFAH1B1 Antibody (N-term)( APR12705G). APR12705G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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