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DHFR Antibody (N-term)

APR07558G Product Datasheet Protocol

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APR07558G		100 μl / $495

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Leading Biology Inc.

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DHFR Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR07558G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DHFR antibody (N-term).
Molecular Weight	21453 Da
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Target	This DHFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human DHFR.
Isotype	Rabbit Ig
GeneID	1719
UniProt ID	P00374
Function	Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Summary	Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	IHC-P~~1:25 WB~~1:1000
Synonyms	Dihydrofolate reductase, DHFR
Images	Immunohistochemical analysis of paraffin-embedded H. liver section using DHFR Antibody (N-term)( APR07558G). APR07558G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Immunohistochemical analysis of paraffin-embedded H. brain section using DHFR Antibody (N-term)( APR07558G). APR07558G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysates from 293T, Hela, Jurkat, Y79 cell line , mouse spleen and rat kidney tissue lysate(from left to right), using DHFR Antibody (N-term)( APR07558G). APR07558G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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