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GLI2 Antibody (C-term)

APG03332G Product Datasheet Protocol

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APG03332G		100 μl / $495

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Leading Biology Inc.

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GLI2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APG03332G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GLI2 antibody (C-term).
Molecular Weight	167783 Da
Cellular Localization	Antigen Cellular Localization: Nucleus {ECO:0000250\|UniProtKB:Q0VGT2}. Cytoplasm {ECO:0000250\|UniProtKB:Q0VGT2}. Note=In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus {ECO:0000250\|UniProtKB:Q0VGT2} Isoform 2: Nucleus
Host	Rabbit
Species Reactivity	Human
Target	This GLI2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1287-1321 amino acids from the C-terminal region of human GLI2.
Isotype	Rabbit Ig
GeneID	2736
UniProt ID	P10070
Function	Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. May play a role during embryogenesis.
Summary	Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Is involved in the smoothened (SHH) signaling pathway.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, IF, E
Dilution	IF~~1:25 FC~~1:25 WB~~1:1000
Synonyms	Zinc finger protein GLI2, GLI family zinc finger protein 2 {ECO:0000312\|HGNC:HGNC:4318}, Tax helper protein, GLI2 (HGNC:4318)
Images	Fluorescent image of Hela cells stained with GLI2 Antibody (C-term)( APG03332G). APG03332G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Flow cytometric analysis of Hela cells using GLI2 Antibody (C-term)(green, APG03332G) compared to an isotype control of rabbit IgG(blue). APG03332G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Western blot analysis of lysates from 293, Jurkat cell line (from left to right), using GLI2 Antibody (C-term)( APG03332G). APG03332G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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